Course Description

The course provides foundation knowledge in molecular biology, genetics, and the molecular basis of selected human diseases. An in-depth review of the theory of molecular techniques such as nucleic acid hybridization, amplification, sequencing, and genotyping is covered. In addition, the application of these techniques in the diagnosis, monitoring, therapeutic decision-making, and prediction of genetic, hematopathologic, infectious, and malignant diseases at the nucleic acid level is discussed. Additional topics include molecular diagnostic laboratory practices and ethical issues.

Credits/Modes of Instruction

Credits:  3

Participants should anticipate spending approximately 135 hours on the course, or an average of 9-12 hours per week for 14 weeks. The course is web-based. Modes of instruction and course format include online instruction, online discussion, and directed study through journal, textbook, Internet readings, and written examinations.

Prerequisites

Baccalaureate degree with coursework in biological sciences and chemistry; previous coursework in molecular biology recommended, but not required.

Course Goals and Outcomes:

Goals

After completion of the course, the student will have 1) foundation knowledge in molecular biology, genetics and the molecular basis of selected diseases, 2) an in-depth understanding of molecular techniques such as nucleic acid hybridization, amplification, sequencing and genotyping, 3) sound understanding of the practical applications of the use of molecular techniques in the diagnosis, monitoring, therapeutic decision-making and prediction of genetic, hematopathologic, infectious, and malignant diseases, and 4) a basic understanding of molecular diagnostic laboratory regulations and practices, and 5) opportunities for discussion of ethical, legal, social, and economical issues relevant to molecular diagnostic testing, as well as future directions in the field.

Describe nucleic acid chemistry, structure and function.
Explain the principle of nucleic acid procedures including nucleic acid extraction and purification, hybridization, amplification and DNA sequencing and genotyping techniques.
Interpret results of selected molecular diagnostic procedures.
Describe concepts in genetics required for interpretation of molecular techniques
Discuss how molecular diagnostic techniques are used in the diagnosis, monitoring, therapeutic decision-making, and prediction of genetic, hematopathologic, infectious, and malignant diseases.
Discuss the appropriate use of molecular techniques in selected situations.
Describe appropriate laboratory regulations and practices required in a molecular diagnostic laboratory.
Discuss how knowledge of the human genome sequence will impact the treatment of diseases.
Discuss ethical, legal, social, and economic issues related to molecular testing.